By Regula M. Keller, Kurt Wüthrich (auth.), Lawrence J. Berliner, Jacques Reuben (eds.)
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Extra info for Biological Magnetic Resonance: Volume 3
For long irradiation times the TOE difference spectra tend toward the steady-state spectrum of Figure 16C. The data of both Figure 17 and Figure 19 are a reliable manifestation of the distances between irradiated and observed protons (Gordon and Wuthrich, 1978; Wagner and Wuthrich, 1979). Compared with transient NOEs, TOEs have important advantages in practical use. One is that the 24 Regula M. KeUer and Kurt Wiithrich amplitude of the preirradiation field can be considerably smaller, so that more selective irradiation in crowded spectral regions may b~ obtained.
27 Multiple Irradiation IH NMR Experiments 3. STUDIES OF THE HEME GROUPS AND THE AXIAL LIGANDS OF THE HEME IRON Observation of the resonances of the heme and the axial ligands of the heme iron in hemoproteins is an important source of data on heme-polypeptide interactions and on the electronic wave functions in the hemes of paramagnetic hemoproteins. The information content of these spectral features is greatly enhanced when individual resonance lines are assigned to specific positions on the heme.
08 ppm was simultaneously decoupled. Below trace (A), the spin systems of Phe-6 and Tyr V, which have not been assigned previously, are indicated. 44 Regula M. 5 6 Figure 30. 1 H NMR studies of the spin system of the partially immobilized ring of Phe-6 in ferrocytochrome c-552 (see also Figure 29). 0, T = 26°C. 32 ppm (filled arrows). 3. Survey of Results Obtained for c-Type Cytochromes As we have seen in the foregoing discussions, the aromatic rings are accessible to particularly extensive description of conformation-dependent properties.